Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637900/GTX637900_T-44872_20230616_WB_R_brain_23062019_203.webp "Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637900/GTX637900_45103_20230721_WB_M_brain_23072519_799.webp "Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637900/GTX637900_45103_20230915_WB_Z_23091901_821.webp "Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:7500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637900/GTX637900_45103_20231013_WB_C_23101921_102.webp "Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:7500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Wnt2 antibody [HL1999] detects Wnt2 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt2 stained by Wnt2 antibody [HL1999] (GTX637900) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637900/GTX637900_45103_20231006_ICC_IF_B_Wnt2_23102401_568.webp "Wnt2 antibody [HL1999] detects Wnt2 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt2 stained by Wnt2 antibody [HL1999] (GTX637900) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (GTX637900) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Wnt2 antibody [HL1999]
GTX637900
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityFeline, Human, Mouse, Rat, Zebra Fish
TargetWNT2
Overview
- SupplierGeneTex
- Product NameWnt2 antibody [HL1999]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1999
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7472
- Target nameWNT2
- Target descriptionWnt family member 2
- Target synonymsINT1L1, IRP, protein Wnt-2, Int-1-related protein, epididymis secretory sperm binding protein, int-1-like protein 1, secreted growth factor, wingless-type MMTV integration site family member 2
- HostRabbit
- IsotypeIgG
- Protein IDP09544
- Protein NameProtein Wnt-2
- Scientific DescriptionThis gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
- ReactivityFeline, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
Related products
Product group Antibodies
Anti-WNT2 Antibody144-66018
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetWNT2
- SizePrice
Product group Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetWNT2
- SizePrice
Product group Antibodies
WNT2 Polyclonal AntibodyBS-6133R
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetWNT2
- SizePrice
Product group Antibodies
WNT2 / IRP AntibodyLS-C771834
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetWNT2
- SizePrice
Product group Antibodies
WNT2 AntibodyCSB-PA026133ESR1HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetWNT2
- SizePrice
Product group Antibodies
WNT2 AntibodyPACO20917
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetWNT2
- SizePrice
Product group Antibodies
Wnt2 antibodyGTX128098
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetWNT2
- SizePrice
Product group Antibodies
Anti-WNT2 AntibodyA31099
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
- SizePrice
Product group Antibodies
Anti-Wnt2 Antibody Picoband(r)PB9461-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman
TargetWNT2
- SizePrice