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ProductsBack/Wnt2 antibody [HL1999]

Wnt2 antibody [HL1999]

GTX637900
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetWNT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Wnt2 antibody [HL1999]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1999
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7472
  • Target name
    WNT2
  • Target description
    Wnt family member 2
  • Target synonyms
    epididymis secretory sperm binding protein; INT1L1; int-1-like protein 1; Int-1-related protein; IRP; protein Wnt-2; secreted growth factor; wingless-type MMTV integration site family member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09544
  • Protein Name
    Protein Wnt-2
  • Scientific Description
    This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WNT2 using anti-WNT2 antibody (PB9461). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: COLO320 Whole Cell Lysate at 40ug, Lane 2: MCF-7 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT2 antigen affinity purified polyclonal antibody (Catalog # PB9461) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT2 at approximately 40 kDa. The expected band size for WNT2 is at 40 kDa.
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