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ProductsBack/WNT3 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3. Positive lysates LY403082 (100ug) and LC403082 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3. Positive lysates LY403082 (100ug) and LC403082 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3. Positive lysates LY403082 (100ug) and LC403082 (20ug) can be purchased separately from OriGene.

WNT3 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D4]

TA801865BM
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetWNT3
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    WNT3 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D4]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2D4
  • Gene ID7473
  • Target name
    WNT3
  • Target description
    Wnt family member 3
  • Target synonyms
    INT4, TETAMS, proto-oncogene Wnt-3, WNT-3 proto-oncogene protein, proto-oncogene Int-4 homolog, wingless-type MMTV integration site family, member 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP56703
  • Protein Name
    Proto-oncogene Wnt-3
  • Scientific Description
    WNT3 mouse monoclonal antibody,clone 2D4, HRP conjugated
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of WNT3 using anti-WNT3 antibody (PB9619). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: HEPG2 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT3 antigen affinity purified polyclonal antibody (Catalog # PB9619) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT3 at approximately 45 kDa. The expected band size for WNT3 is at 45 kDa.
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