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WNT8B / Wnt 8b Antibody

LS-C40267
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetWNT8B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    WNT8B / Wnt 8b Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Immunohistochemistry and Direct ELISA.
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (0.5 - 1 µg/ml), IHC, IHC-Pfa (10 µg/ml) Suitable for use in Immunohistochemistry and Direct ELISA.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7479
  • Target name
    WNT8B
  • Target description
    Wnt family member 8B
  • Target synonyms
    protein Wnt-8b, epididymis secretory sperm binding protein, wingless-type MMTV integration site family, member 8B
  • Host
    Rat
  • Isotype
    IgG2a
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C or -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WNT8B using anti-WNT8B antibody (A07933-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT8B antigen affinity purified polyclonal antibody (Catalog # A07933-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT8B at approximately 40-50 kDa. The expected band size for WNT8B is at 39 kDa.
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