WNT8B / Wnt 8b Antibody

LifeSpan BioSciences

WNT8B / Wnt 8b Antibody

23

Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit for rmWnt-8b is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. This antibody was used at 5 ug/ml to detect Wnt-8b in cell extracts. Western Blot buffers are listed below. Wash Buffer Blocking Buffer Blotting Buffer 25 mM Tris, pH 7.4 5% nonfat dry milk 2% nonfat dry milk. 0.5 M NaCl in Wash Buffer in Wash Buffer 0.05% Tween 20. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. The detection limit for rmWnt-8b is approximately 5 ng/well.. ELISA, WB Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit for rmWnt-8b is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. This antibody was used at 5 ug/ml to detect Wnt-8b in cell extracts. Western Blot buffers are listed below. Wash Buffer Blocking Buffer Blotting Buffer 25 mM Tris, pH 7.4 5% nonfat dry milk 2% nonfat dry milk. 0.5 M NaCl in Wash Buffer in Wash Buffer 0.05% Tween 20. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. The detection limit for rmWnt-8b is approximately 5 ng/well.

Western Blot, ELISA

Research Use Only

Monoclonal

Unconjugated

...

WNT8B

Wnt family member 8B

epididymis secretory sperm binding protein; protein Wnt-8b; wingless-type MMTV integration site family, member 8B

Rat

IgG2a

12352203