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ProductsBack/XCR1 Antibody (aa244-322)

XCR1 Antibody (aa244-322)

LS-C763561
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetXCR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    XCR1 Antibody (aa244-322)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA 0.05-2ug/ml; ICC 5-20ug/ml; WB 0.5-2ug/ml;
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:50 - 1:400) ELISA 0.05-2ug/ml; ICC 5-20ug/ml; WB 0.5-2ug/ml;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2829
  • Target name
    XCR1
  • Target description
    X-C motif chemokine receptor 1
  • Target synonyms
    CCXCR1, GPR5, chemokine XC receptor 1, G protein-coupled receptor 5, XC chemokine receptor 1, chemokine (C motif) XC receptor 1, chemokine (C motif) receptor 1, lymphotactin receptor
  • Host
    Rabbit
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (A04185-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCXCR1/XCR1 antigen affinity purified polyclonal antibody (Catalog # A04185-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCXCR1/XCR1 at approximately 42 kDa. The expected band size for CCXCR1/XCR1 is at 39 kDa.
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