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ProductsBack/XLF antibody [1G7]
FACS analysis of HeLa cells using GTX84043 XLF antibody [1G7]. Red : Primary antibody Blue : Negative control antibody
FACS analysis of HeLa cells using GTX84043 XLF antibody [1G7]. Red : Primary antibody Blue : Negative control antibody
FACS analysis of HeLa cells using GTX84043 XLF antibody [1G7]. Red : Primary antibody Blue : Negative control antibody

XLF antibody [1G7]

GTX84043
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNHEJ1
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Overview

  • Supplier
    GeneTex
  • Product Name
    XLF antibody [1G7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1g7
  • Concentration
    0.66 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID79840
  • Target name
    NHEJ1
  • Target description
    non-homologous end joining factor 1
  • Target synonyms
    IMD124, MCOPCB13, XLF, non-homologous end-joining factor 1, XRCC4-like factor, nonhomologous end-joining factor 1, protein cernunnos
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9H9Q4
  • Protein Name
    Non-homologous end-joining factor 1
  • Scientific Description
    DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NHEJ1 using anti-NHEJ1 antibody (A03552-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NHEJ1 antigen affinity purified polyclonal antibody (Catalog # A03552-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NHEJ1 at approximately 38KD. The expected band size for NHEJ1 is at 33KD.
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