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Western Blot analysis of COLO205 cells using XPA Polyclonal Antibody
Western Blot analysis of COLO205 cells using XPA Polyclonal Antibody
Western Blot analysis of COLO205 cells using XPA Polyclonal Antibody

XPA Antibody

CSB-PA004520
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetXPA
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Overview

  • Supplier
    Cusabio
  • Product Name
    XPA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7507
  • Target name
    XPA
  • Target description
    XPA, DNA damage recognition and repair factor
  • Target synonyms
    DNA repair protein complementing XP-A cells; xeroderma pigmentosum group A-complementing protein; xeroderma pigmentosum, complementation group A; XP1; XPAC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23025
  • Protein Name
    DNA repair protein complementing XP-A cells
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of XPA using anti-XPA antibody (A01182-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPA antigen affinity purified polyclonal antibody (Catalog # A01182-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPA at approximately 39 kDa. The expected band size for XPA is at 31 kDa.
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