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ProductsBack/XRCC1 Mouse Monoclonal Antibody [Clone ID: OTI2G8]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1 (1:1000).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1 (1:1000).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1 (1:1000).

XRCC1 Mouse Monoclonal Antibody [Clone ID: OTI2G8]

CF500879
OriGene
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetXRCC1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    XRCC1 Mouse Monoclonal Antibody [Clone ID: OTI2G8]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2G8
  • Gene ID7515
  • Target name
    XRCC1
  • Target description
    X-ray repair cross complementing 1
  • Target synonyms
    RCC, SCAR26, DNA repair protein XRCC1, X-ray repair complementing defective repair in Chinese hamster cells 1, X-ray repair cross-complementing protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP18887
  • Protein Name
    DNA repair protein XRCC1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) XRCC1 mouse monoclonal antibody, clone OTI2G8 (formerly 2G8)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 5. IHC analysis of XRCC1 using anti-XRCC1 antibody (A00571). XRCC1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-XRCC1 Antibody (A00571) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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