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Western Blot analysis of 293 cells using XRCC2 Polyclonal Antibody
Western Blot analysis of 293 cells using XRCC2 Polyclonal Antibody
Western Blot analysis of 293 cells using XRCC2 Polyclonal Antibody

XRCC2 Antibody

CSB-PA004530
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetXRCC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    XRCC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7516
  • Target name
    XRCC2
  • Target description
    X-ray repair cross complementing 2
  • Target synonyms
    DNA repair protein XRCC2; epididymis secretory sperm binding protein; FANCU; POF17; SPGF50; X-ray repair complementing defective repair in Chinese hamster cells 2; X-ray repair cross-complementing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43543
  • Protein Name
    DNA repair protein XRCC2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of XRCC2 using anti-XRCC2 antibody (A02138-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC2 antigen affinity purified polyclonal antibody (Catalog # A02138-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC2 at approximately 32KD. The expected band size for XRCC2 is at 32KD.
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