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XRCC2 antibody [N1N3]

GTX100113
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetXRCC2
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Overview

  • Supplier
    GeneTex
  • Product Name
    XRCC2 antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7516
  • Target name
    XRCC2
  • Target description
    X-ray repair cross complementing 2
  • Target synonyms
    DNA repair protein XRCC2; epididymis secretory sperm binding protein; FANCU; POF17; SPGF50; X-ray repair complementing defective repair in Chinese hamster cells 2; X-ray repair cross-complementing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43543
  • Protein Name
    DNA repair protein XRCC2
  • Scientific Description
    This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of XRCC2 using anti-XRCC2 antibody (A02138-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC2 antigen affinity purified polyclonal antibody (Catalog # A02138-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC2 at approximately 32KD. The expected band size for XRCC2 is at 32KD.
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