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XRCC4 Antibody (YA1082, Mouse)

HY-P81337
MedChem Express
TargetXRCC4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    XRCC4 Antibody (YA1082, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7518
  • Target name
    XRCC4
  • Target description
    X-ray repair cross complementing 4
  • Target synonyms
    SSMED, hXRCC4, DNA repair protein XRCC4, X-ray repair complementing defective repair in Chinese hamster cells 4
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    XRCC4 Antibody (YA1082) is a mouse-derived non-conjugated IgG1 antibody (Clone NO.: YA1082), targeting XRCC4, with a predicted molecular weight of 38 kDa (observed band size: 38-45 kDa). XRCC4 Antibody (YA1082) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human, mouse, rat background.
  • UNSPSC
    41116161
  • Species
    Mouse

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Figure 1. Western blot analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC4 antigen affinity purified polyclonal antibody (Catalog # A00787-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC4 at approximately 55 kDa. The expected band size for XRCC4 is at 38 kDa.
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