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Western Blot analysis of HepG2 cells using YAP Polyclonal Antibody
Western Blot analysis of HepG2 cells using YAP Polyclonal Antibody
Western Blot analysis of HepG2 cells using YAP Polyclonal Antibody

YAP1 Antibody

CSB-PA004537
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetYAP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    YAP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10413
  • Target name
    YAP1
  • Target description
    Yes1 associated transcriptional regulator
  • Target synonyms
    65 kDa Yes-associated protein; COB1; protein yorkie homolog; transcriptional coactivator YAP1; YAP; YAP2; YAP65; Yes associated protein 1; yes-associated protein 2; yes-associated protein YAP65 homolog; YKI; yorkie homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46937
  • Protein Name
    Transcriptional coactivator YAP1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of YAP1 using anti-YAP1 antibody (PB9967). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (Catalog # PB9967) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YAP1 at approximately 70 kDa. The expected band size for YAP1 is at 54 kDa.
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