
ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04728 YAP1 (phospho Ser127) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1 μg/mL
YAP1 (phospho Ser127) antibody
GTX04728
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetYAP1
Overview
- SupplierGeneTex
- Product NameYAP1 (phospho Ser127) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ICC/IF: 1:100-1:500. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10413
- Target nameYAP1
- Target descriptionYes1 associated transcriptional regulator
- Target synonymsCOB1, YAP, YAP-1, YAP2, YAP65, YKI, transcriptional coactivator YAP1, 65 kDa Yes-associated protein, Yes associated protein 1, protein yorkie homolog, yes-associated protein 2, yes-associated protein YAP65 homolog, yorkie homolog
- HostRabbit
- IsotypeIgG
- Protein IDP46937
- Protein NameTranscriptional coactivator YAP1
- Scientific DescriptionThis gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203



![YAP1 antibody immunoprecipitates YAP1 protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of YAP1 protein by 4 μg YAP1 antibody (GTX129151) 7.5 % SDS-PAGE The immunoprecipitated YAP1 protein was detected by YAP1 antibody (GTX129151) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX129151/GTX129151_41500_IP_w_23060523_133.webp)


![WB analysis of HeLa (1), C6 (2) and Cos7 (3) cell lysate using GTX60554 YAP1 antibody [1A12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60554/GTX60554_20170912_WB_w_23061123_663.webp)
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody [GT256] (GTX633541) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633541/GTX633541_42520_20160922_WB_shRNA_watermark_w_23061202_151.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637643/GTX637643_T-44837_20221111_WB_22110919_573.webp)
