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YARS / Tyrosyl-tRNA Synthetase Antibody

LS-C813572
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetYARS1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    YARS / Tyrosyl-tRNA Synthetase Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8565
  • Target name
    YARS1
  • Target description
    tyrosyl-tRNA synthetase 1
  • Target synonyms
    CMTDIC; IMNEPD2; tyrosine tRNA ligase 1, cytoplasmic; tyrosine--tRNA ligase, cytoplasmic; tyrosyl--tRNA ligase; tyrosyl-tRNA synthetase, cytoplasmic; TYRRS; YARS; YRS; YTS
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of YARS1 using anti-YARS1 antibody (A08583-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YARS1 antigen affinity purified polyclonal antibody (Catalog # A08583-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YARS1 at approximately 59 kDa. The expected band size for YARS1 is at 59 kDa.
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