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Western Blot analysis of HeLa cells using MLTK Polyclonal Antibody
Western Blot analysis of HeLa cells using MLTK Polyclonal Antibody
Western Blot analysis of HeLa cells using MLTK Polyclonal Antibody

ZAK Antibody

CSB-PA003251
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMAP3K20
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Overview

  • Supplier
    Cusabio
  • Product Name
    ZAK Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51776
  • Target name
    MAP3K20
  • Target description
    mitogen-activated protein kinase kinase kinase 20
  • Target synonyms
    AZK, CNM6, MLK7, MLT, MLTK, MLTKalpha, MLTKbeta, MRK, SFMMP, ZAK, mlklak, pk, mitogen-activated protein kinase kinase kinase 20, HCCS-4, MLK-like mitogen-activated protein triple kinase, ZAK1 homolog, leucine zipper and sterile-alpha motif kinase, human cervical cancer suppressor gene 4 protein, mitogen-activated protein kinase kinase kinase MLT, mixed lineage kinase 7, mixed lineage kinase with a leucine zipper and a sterile alpha motif, mixed lineage kinase-related kinase MRK-beta, sterile alpha motif and leucine zipper containing kinase AZK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYL2
  • Protein Name
    Mitogen-activated protein kinase kinase kinase 20
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAP3K20 using anti-MAP3K20 antibody (A00902-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K20 antigen affinity purified polyclonal antibody (Catalog # A00902-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP3K20 at approximately 91KD. The expected band size for MAP3K20 is at 91KD.
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Wild-type (WT) and ZAK knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAK antibody [N3C2], Internal (GTX103317) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
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ZAK antibody [N3C2], InternalGTX103317
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TargetMAP3K20
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