![Wild-type (WT) and ZAK knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAK antibody [N3C2], Internal (GTX103317) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Wild-type (WT) and ZAK knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAK antibody [N3C2], Internal (GTX103317) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103317/GTX103317_40079_20181123_WB_KO_watermark_w_23060119_470.webp)
Wild-type (WT) and ZAK knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAK antibody [N3C2], Internal (GTX103317) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
ZAK antibody [N3C2], Internal
GTX103317
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMAP3K20
Overview
- SupplierGeneTex
- Product NameZAK antibody [N3C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.92 mg/ml
- ConjugateUnconjugated
- Gene ID51776
- Target nameMAP3K20
- Target descriptionmitogen-activated protein kinase kinase kinase 20
- Target synonymsAZK, CNM6, MLK7, MLT, MLTK, MLTKalpha, MLTKbeta, MRK, SFMMP, ZAK, mlklak, pk, mitogen-activated protein kinase kinase kinase 20, HCCS-4, MLK-like mitogen-activated protein triple kinase, ZAK1 homolog, leucine zipper and sterile-alpha motif kinase, human cervical cancer suppressor gene 4 protein, mitogen-activated protein kinase kinase kinase MLT, mixed lineage kinase 7, mixed lineage kinase with a leucine zipper and a sterile alpha motif, mixed lineage kinase-related kinase MRK-beta, sterile alpha motif and leucine zipper containing kinase AZK
- HostRabbit
- IsotypeIgG
- Protein IDQ9NYL2
- Protein NameMitogen-activated protein kinase kinase kinase 20
- Scientific DescriptionThis gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203





![ZAK antibody [N1N2], N-term detects ZAK protein at on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. ZAK antibody [N1N2], N-term (GTX111477) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111477/GTX111477_40065_IHC_w_23060500_428.webp)