IP analysis of Jurkat cell lysates (ZAP-70 positive expression cell line) and Ramos cell lysates (ZAP-70 negative expression cell line) using GTX66846 ZAP70 antibody [4B2].
![WB analysis of CEM, Jurkat cell, and Ramos cell lysates (negative control) using GTX66846 ZAP70 antibody [4B2]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX66846/GTX66846_20190305_WB_w_23061221_853.webp "WB analysis of CEM, Jurkat cell, and Ramos cell lysates (negative control) using GTX66846 ZAP70 antibody [4B2]. Dilution : 1:1000")
IP analysis of Jurkat cell lysates (ZAP-70 positive expression cell line) and Ramos cell lysates (ZAP-70 negative expression cell line) using GTX66846 ZAP70 antibody [4B2].
ZAP70 antibody [4B2]
GTX66846
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetZAP70
Overview
- SupplierGeneTex
- Product NameZAP70 antibody [4B2] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. IP: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4B2
- ConjugateUnconjugated
- Gene ID7535
- Target nameZAP70
- Target descriptionzeta chain of T cell receptor associated protein kinase 70
- Target synonyms70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; IMD48; SRK; STCD; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
- HostMouse
- IsotypeIgG
- Protein IDP43403
- Protein NameTyrosine-protein kinase ZAP-70
- Scientific DescriptionThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203