Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636914/GTX636914_44697_20221216_WB_C_22122018_487.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636914/GTX636914_44697_20220610_WB_treatment_LPS_w_23061202_434.webp "Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 | 20 | 20 | 10 | 10 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636914/GTX636914_44697_20230714_WB_multiple_B_23071822_994.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 | 20 | 20 | 10 | 10 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
ZC3H12A antibody [HL1442]
GTX636914
ApplicationsWestern Blot
Product group Antibodies
ReactivityFeline, Human
TargetZC3H12A
Overview
- SupplierGeneTex
- Product NameZC3H12A antibody [HL1442]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1442
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID80149
- Target nameZC3H12A
- Target descriptionzinc finger CCCH-type containing 12A
- Target synonymsMCPIP, MCPIP-1, MCPIP1, Reg1, dJ423B22.1, endoribonuclease ZC3H12A, MCP-1 treatment-induced protein, MCP-induced protein 1, bifunctional endoribonuclease and deubiquitinase ZC3H12A, monocyte chemotactic protein-induced protein 1, regnase-1, ribonuclease ZC3H12A, zinc finger CCCH domain-containing protein 12A
- HostRabbit
- IsotypeIgG
- Protein IDQ5D1E8
- Protein NameEndoribonuclease ZC3H12A
- Scientific DescriptionZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM, Mar 2008]
- ReactivityFeline, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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