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ProductsBack/ZCCHC5 Antibody - N-terminal region (OAAB00047)

ZCCHC5 Antibody - N-terminal region (OAAB00047)

OAAB00047
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetRTL3
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    ZCCHC5 Antibody - N-terminal region (OAAB00047)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Gene ID203430
  • Target name
    RTL3
  • Target description
    retrotransposon Gag like 3
  • Target synonyms
    Mar3, Mart3, SIRH9, ZCCHC5, ZHC5, retrotransposon Gag-like protein 3, Sushi-Ichi retrotransposon homolog 9, mammalian retrotransposon-derived 3, zinc finger CCHC domain-containing protein 5, zinc finger CCHC-type containing 5, zinc finger, CCHC domain containing 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RTL3 using anti-RTL3 antibody (A31819). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTL3 antigen affinity purified polyclonal antibody (Catalog # A31819) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTL3 at approximately 50 kDa. The expected band size for RTL3 is at 53 kDa.
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