Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638294/GTX638294_T-44960_20230317_WB_shRNA_watermark_23032022_972.webp "Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ZEB1 stained by ZEB1 antibody [HL2245] (GTX638294) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638294/GTX638294_T-44960_20230325_IHC-P_multiple_R_23032819_618.webp "ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ZEB1 stained by ZEB1 antibody [HL2245] (GTX638294) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ZEB1 stained by ZEB1 antibody [HL2245] (GTX638294) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638294/GTX638294_T-44960_20230325_IHC-P_multiple_M_23032819_632.webp "ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ZEB1 stained by ZEB1 antibody [HL2245] (GTX638294) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 stained by ZEB1 antibody [HL2245] (GTX638294) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638294/GTX638294_T-44960_20230331_ICC_IF_23041023_765.webp "ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 stained by ZEB1 antibody [HL2245] (GTX638294) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638294/GTX638294_45026_20230428_WB_TPM_watermark_23050223_402.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![(30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638294/GTX638294_45026_20230707_WB_TPM_watermark_23071223_447.webp "(30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638294/GTX638294_45026_20230707_WB_competitor_watermark_23071223_635.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ZEB1 antibody [HL2245]
GTX638294
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetZEB1
Overview
- SupplierGeneTex
- Product NameZEB1 antibody [HL2245]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2245
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6935
- Target nameZEB1
- Target descriptionzinc finger E-box binding homeobox 1
- Target synonymsAREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A, zinc finger E-box-binding homeobox 1, delta-crystallin enhancer binding factor 1, negative regulator of IL2, posterior polymorphous corneal dystrophy 3, transcription factor 8 (represses interleukin 2 expression), zinc finger homeodomain enhancer-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP37275
- Protein NameZinc finger E-box-binding homeobox 1
- Scientific DescriptionThis gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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