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ProductsBack/ZEB2 / SIP-1 Antibody

ZEB2 / SIP-1 Antibody

LS-C401022
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetZEB2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ZEB2 / SIP-1 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9839
  • Target name
    ZEB2
  • Target description
    zinc finger E-box binding homeobox 2
  • Target synonyms
    HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B, zinc finger E-box-binding homeobox 2, SMAD interacting protein 1, Smad-interacting protein 1, zinc finger homeobox 1b
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 3. Flow Cytometry analysis of U937 cells using anti-ZEB2 antibody (PB9236). Overlay histogram showing U937 cells stained with PB9236 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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