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ZFP42 Antibody (YA4433, Mouse)

HY-P84736
MedChem Express
TargetZFP42
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ZFP42 Antibody (YA4433, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID132625
  • Target name
    ZFP42
  • Target description
    ZFP42 zinc finger protein
  • Target synonyms
    reduced expression protein 1; REX1; REX-1; REX1 transcription factor; zfp-42; zinc finger protein 42 homolog; zinc finger protein 754; ZNF754
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    ZFP42 Antibody (YA4433) is a mouse-derived and non-conjugated IgG1 antibody, targeting to ZFP42. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, mouse.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Rex1/ZFP42 using anti-Rex1/ZFP42 antibody (A08177-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rex1/ZFP42 antigen affinity purified polyclonal antibody (Catalog # A08177-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rex1/ZFP42 at approximately 45KD. The expected band size for Rex1/ZFP42 is at 45KD.
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