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Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-3: A549 cells, K562 cells, PC3 cells, Primary antibody: CSB-PA238285(ZFP42 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-3: A549 cells, K562 cells, PC3 cells, Primary antibody: CSB-PA238285(ZFP42 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-3: A549 cells, K562 cells, PC3 cells, Primary antibody: CSB-PA238285(ZFP42 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds

ZFP42 Antibody

CSB-PA238285
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetZFP42
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Overview

  • Supplier
    Cusabio
  • Product Name
    ZFP42 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID132625
  • Target name
    ZFP42
  • Target description
    ZFP42 zinc finger protein
  • Target synonyms
    reduced expression protein 1; REX1; REX-1; REX1 transcription factor; zfp-42; zinc finger protein 42 homolog; zinc finger protein 754; ZNF754
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96MM3
  • Protein Name
    Zinc finger protein 42 homolog
  • Scientific Description
    Rex-1 (for reduced expression), also designated zinc finger protein 42 (ZFP42), is an acidic zinc finger protein. Rex-1 contains four repeats of the zinc finger nucleic acid-binding motif and a potential acidic activator domain, suggesting that it is a regulatory protein. Rex-1 localizes to the nucleus and is highly expressed in embryonic stem (ES) and undifferentiated murine F9 teratocarcinoma cells. At the transcriptional level, expression of Rex-1 is reduced when F9 cells are induced to differentiate by the addition of retinoic acid (RA), and Rex-1 repression is enhanced by E1A. The Oct-3/4 transcription factor can either activate or repress the Rex-1 promoter, depending on the cellular environment, while Oct-6 can lower the expression of Rex-1.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Rex1/ZFP42 using anti-Rex1/ZFP42 antibody (A08177-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rex1/ZFP42 antigen affinity purified polyclonal antibody (Catalog # A08177-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rex1/ZFP42 at approximately 45KD. The expected band size for Rex1/ZFP42 is at 45KD.
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