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ProductsBack/ZNF318 Polyclonal Antibody
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Testis lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HL-60 cell lysates; Lane 5: Human 293T cell lysates probed with ZNF318 Polyclonal Antibody, Unconjugated (bs-11414R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Testis lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HL-60 cell lysates; Lane 5: Human 293T cell lysates probed with ZNF318 Polyclonal Antibody, Unconjugated (bs-11414R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Testis lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HL-60 cell lysates; Lane 5: Human 293T cell lysates probed with ZNF318 Polyclonal Antibody, Unconjugated (bs-11414R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

ZNF318 Polyclonal Antibody

BS-11414R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetZNF318
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Overview

  • Supplier
    Bioss
  • Product Name
    ZNF318 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID24149
  • Target name
    ZNF318
  • Target description
    zinc finger protein 318
  • Target synonyms
    HRIHFB2436, TZF, ZFP318, zinc finger protein 318, endocrine regulator, endocrine regulatory protein, testicular zinc finger
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZNF318 using anti-ZNF318 antibody (A11267-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat stomach tissue lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNF318 antigen affinity purified polyclonal antibody (Catalog # A11267-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZNF318 at approximately 290 kDa. The expected band size for ZNF318 is at 251 kDa.
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