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Immunohistochemical staining of human testis shows distinct nuclear positivity in seminiferous duct cells.
Immunohistochemical staining of human testis shows distinct nuclear positivity in seminiferous duct cells.
Immunohistochemical staining of human testis shows distinct nuclear positivity in seminiferous duct cells.

Anti-ZNF318 Antibody

HPA027022
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZNF318
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZNF318 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID24149
  • Target name
    ZNF318
  • Target description
    zinc finger protein 318
  • Target synonyms
    HRIHFB2436, TZF, ZFP318, zinc finger protein 318, endocrine regulator, endocrine regulatory protein, testicular zinc finger
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VUA4
  • Protein Name
    Zinc finger protein 318
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Testis lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HL-60 cell lysates; Lane 5: Human 293T cell lysates probed with ZNF318 Polyclonal Antibody, Unconjugated (bs-11414R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss
ZNF318 Polyclonal AntibodyBS-11414R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetZNF318
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Figure 1. Western blot analysis of ZNF318 using anti-ZNF318 antibody (A11267-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat stomach tissue lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNF318 antigen affinity purified polyclonal antibody (Catalog # A11267-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZNF318 at approximately 290 kDa. The expected band size for ZNF318 is at 251 kDa.
Product group Antibodies
Boster Bio
Anti-ZNF318 Antibody Picoband(r)A11267-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetZNF318
  • SizePrice
Atlas Antibodies
PrEST Antigen ZNF318APREST73744
Protein IDQ5VUA4
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PrEST Antigen ZNF318APREST73745
Protein IDQ5VUA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ZNF318 Antibody (aa2049-2099)LS-C286552
ApplicationsImmunoFluorescence, ImmunoHistoChemistry
TargetZNF318
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Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-ZNF318 AntibodyHPA027031
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetZNF318
  • SizePrice