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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA030465(ZWINT Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA030465(ZWINT Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA030465(ZWINT Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)

ZWINT Antibody

CSB-PA030465
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetZWINT
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Overview

  • Supplier
    Cusabio
  • Product Name
    ZWINT Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11130
  • Target name
    ZWINT
  • Target description
    ZW10 interacting kinetochore protein
  • Target synonyms
    human ZW10 interacting protein-1; HZwint-1; KNTC2AP; SIP30; SNAP25 interacting protein of 30 kDa; ZW10 interactor; ZW10 interactor, kinetochore protein; ZWINT1; zwint-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95229
  • Protein Name
    ZW10 interactor
  • Scientific Description
    This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZWINT using anti-ZWINT antibody (PB10018). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody (Catalog # PB10018) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZWINT at approximately 31 kDa. The expected band size for ZWINT is at 31 kDa.
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