
14-3-3 sigma antibody detects SFN protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract 12% SDS-PAGE 14-3-3 sigma antibody (GTX100801) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
14-3-3 sigma antibody
GTX100801
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSFN
Overview
- SupplierGeneTex
- Product Name14-3-3 sigma antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.32 mg/ml
- ConjugateUnconjugated
- Gene ID2810
- Target nameSFN
- Target descriptionstratifin
- Target synonymsYWHAS, 14-3-3 protein sigma, epithelial cell marker protein 1
- HostRabbit
- IsotypeIgG
- Protein IDP31947
- Protein Name14-3-3 protein sigma
- Scientific DescriptionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Wang C, Zhai SN, Yuan XG, et al. Common differentially expressed proteins were found in mouse cleft palate models induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and retinoic acid. Environ Toxicol Pharmacol. 2019,72:103270. doi: 10.1016/j.etap.2019.103270Read this paper
- Wu JY, Lin SS, Hsu FT, et al. Fluoxetine Inhibits DNA Repair and NF-ĸB-modulated Metastatic Potential in Non-small Cell Lung Cancer. Anticancer Res. 2018,38(9):5201-5210. doi: 10.21873/anticanres.12843Read this paper
- Kim M, Morales LD, Baek M, et al. UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation. Oncotarget. 2017,8(53):90674-90692. doi: 10.18632/oncotarget.21794Read this paper
- Li Z, Han S, Wang X, et al. Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium. Stem Cell Res Ther. 2015,6(1):17. doi: 10.1186/s13287-015-0008-2Read this paper
- Sinanoglu O, Yener AN, Ekici S, et al. The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats. Urology. 2012,80(6):1392.e1-6. doi: 10.1016/j.urology.2012.06.053Read this paper
- Tseng HL, Li CJ, Huang LH, et al. Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway. Toxicol Appl Pharmacol. 2012,264(1):104-13. doi: 10.1016/j.taap.2012.07.022Read this paper

![14-3-3 sigma antibody immunoprecipitates SFN protein in IP experiments. IP samples: A431 whole cell extract A. 40 μg A431 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of SFN protein by 4 μg 14-3-3 sigma antibody (GTX100801) 5 % SDS-PAGE The immunoprecipitated SFN protein was detected by 14-3-3 sigma antibody (GTX100801) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent] 14-3-3 sigma antibody immunoprecipitates SFN protein in IP experiments. IP samples: A431 whole cell extract A. 40 μg A431 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of SFN protein by 4 μg 14-3-3 sigma antibody (GTX100801) 5 % SDS-PAGE The immunoprecipitated SFN protein was detected by 14-3-3 sigma antibody (GTX100801) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX100801/GTX100801_40569_IP_w_23060100_315.webp)









![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody [N1C3-2] (GTX112949) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112949/GTX112949_40079_20160616_WB_w_23060500_733.webp)
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 antibody [HL1776] (GTX637428) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637428/GTX637428_45376_20240927_WB_24100318_858.webp)


