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14-3-3 sigma antibody detects SFN protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract 12% SDS-PAGE 14-3-3 sigma antibody (GTX100801) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
14-3-3 sigma antibody detects SFN protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract 12% SDS-PAGE 14-3-3 sigma antibody (GTX100801) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
14-3-3 sigma antibody detects SFN protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract 12% SDS-PAGE 14-3-3 sigma antibody (GTX100801) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

14-3-3 sigma antibody

GTX100801
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSFN
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Overview

  • Supplier
    GeneTex
  • Product Name
    14-3-3 sigma antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.32 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2810
  • Target name
    SFN
  • Target description
    stratifin
  • Target synonyms
    YWHAS, 14-3-3 protein sigma, epithelial cell marker protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31947
  • Protein Name
    14-3-3 protein sigma
  • Scientific Description
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

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  • Wang C, Zhai SN, Yuan XG, et al. Common differentially expressed proteins were found in mouse cleft palate models induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and retinoic acid. Environ Toxicol Pharmacol. 2019,72:103270. doi: 10.1016/j.etap.2019.103270
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  • Kim M, Morales LD, Baek M, et al. UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation. Oncotarget. 2017,8(53):90674-90692. doi: 10.18632/oncotarget.21794
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  • Li Z, Han S, Wang X, et al. Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium. Stem Cell Res Ther. 2015,6(1):17. doi: 10.1186/s13287-015-0008-2
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  • Sinanoglu O, Yener AN, Ekici S, et al. The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats. Urology. 2012,80(6):1392.e1-6. doi: 10.1016/j.urology.2012.06.053
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  • Tseng HL, Li CJ, Huang LH, et al. Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway. Toxicol Appl Pharmacol. 2012,264(1):104-13. doi: 10.1016/j.taap.2012.07.022
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