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ProductsBack/20S Proteasome alpha5 (B-9) Alexa Fluor(r) 488

20S Proteasome alpha5 (B-9) Alexa Fluor(r) 488

SC-137240AF488
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPSMA5
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    20S Proteasome alpha5 (B-9) Alexa Fluor(r) 488
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 488
  • Gene ID5686
  • Target name
    PSMA5
  • Target description
    proteasome 20S subunit alpha 5
  • Target synonyms
    PSC5, ZETA, proteasome subunit alpha type-5, alpha-5, epididymis tissue sperm binding protein Li 11n, macropain subunit zeta, macropain zeta chain, multicatalytic endopeptidase complex zeta chain, proteasome (prosome, macropain) subunit, alpha type, 5, proteasome alpha 5 subunit, proteasome component 5, proteasome subunit alpha 5, proteasome subunit zeta, proteasome zeta chain
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMA5 using anti-PSMA5 antibody (A07499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA5 antigen affinity purified polyclonal antibody (Catalog # A07499-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA5 at approximately 26 kDa. The expected band size for PSMA5 is at 26 kDa.
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