4-Methylumbelliferyl-beta-D-glucopyranoside [18997-57-4] [18997-57-4]

Chemodex

4-Methylumbelliferyl-beta-D-glucopyranoside [18997-57-4] [18997-57-4]

10

18997-57-4

Research Use Only

>97%

C16H18O8

338.31

4-Methylumbelliferyl-beta-D-glucopyranoside (MUD) is a non-fluorescent but sensitive fluorogenic substrate for beta-glucosidase and beta-glucocerebrosidase (also known as glucosylceramidase) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is employed in the detection of beta-glucosidase as an indicator of Enterococci. It has been extensively used in work on Gauchers disease, a lipid storage disease characterized by the accumulation of glucocerebroside due to a genetic deficiency of a beta-glucosidase. The use of 4-Methylumbelliferyl-beta-D-glucopyranoside has also been reported in a rapid method for identifying bacterial enzymes. Hydrolysis of MUD releases the fluorescent product 4-MU. Spectral Data. lambdaEx/lambdaEm= 360/450 nm (cleaved product, pH-dependent). - Chemical. CAS: 18997-57-4. Formula: C16H18O8. MW: 338.31. 4-Methylumbelliferyl-beta-D-glucopyranoside (MUD) is a non-fluorescent but sensitive fluorogenic substrate for beta-glucosidase and beta-glucocerebrosidase (also known as glucosylceramidase) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is employed in the detection of beta-glucosidase as an indicator of Enterococci. It has been extensively used in work on Gauchers disease, a lipid storage disease characterized by the accumulation of glucocerebroside due to a genetic deficiency of a beta-glucosidase. The use of 4-Methylumbelliferyl-beta-D-glucopyranoside has also been reported in a rapid method for identifying bacterial enzymes. Hydrolysis of MUD releases the fluorescent product 4-MU. Spectral Data. lambdaEx/lambdaEm= 360/450 nm (cleaved product, pH-dependent).

O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@@H](O)[C@@H]1O

-20°C,2°C to 8°C

12162000