Detection of 5-Carboxylcytosine (5-caC), in linear denatured DNA (426 bp). Sandwich ELISA using anti-Carboxylcytosine (5-caC) (clone RM462) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP conjugated streptavidin.
Detection of 5-Carboxylcytosine (5-caC), in linear denatured DNA (426 bp). Sandwich ELISA using anti-Carboxylcytosine (5-caC) (clone RM462) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP conjugated streptavidin.
5-Carboxylcytosine (5-caC) DNA Matched Antibody Pair
REV-31-1354-MK
Product group Antibodies
Overview
- SupplierRevMAb Biosciences
- Product Name5-Carboxylcytosine (5-caC) DNA Matched Antibody Pair
- Delivery Days Customer10
- CertificationResearch Use Only
- Scientific Description5-Carboxylcytosine (5-caC) is a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation. - Matched Pair Detection Set. This antibody pair detects 5-Carboxylcytosine (5-caC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203