Detection of 5-Hydroxymethylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Hydroxymethylcytosine (clone RM236) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.
Detection of 5-Hydroxymethylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Hydroxymethylcytosine (clone RM236) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.
5-Hydroxymethylcytosine (5-hmC) DNA Matched Antibody Pair
REV-31-1111-MK
Product group Antibodies
Overview
- SupplierRevMAb Biosciences
- Product Name5-Hydroxymethylcytosine (5-hmC) DNA Matched Antibody Pair
- Delivery Days Customer10
- CertificationResearch Use Only
- Scientific Description5-Hydroxymethylcytosine (5hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC). It not only marks active demethylation but also serves as a relatively stable DNA mark that plays distinct epigenetic roles. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off. 5hmC is abundantly expressed, but the levels vary significantly depending on the cell type. The highest levels are found in neuronal cells of the central nervous system. - Matched Pair Detection Set. This antibody pair detects 5-hydroxymethylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-caC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
