5-Vinyl-2-deoxyuridine (5-VdU), 5-Ethenyl-2-deoxyuridine [55520-67-7]

Jena Bioscience

5-Vinyl-2-deoxyuridine (5-VdU), 5-Ethenyl-2-deoxyuridine [55520-67-7]

5

55520-67-7

Research Use Only

≥98%

C11H14N2O5

254.24 g/mol

5-VdU (5-Vinyl-2-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging [1].

n1(cc(c(=O)[nH]c1=O)C=C)[C@H]1C[C@@H]([C@H](O1)CO)O

-20°C

12352200