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67kDa Laminin Receptor Antibody (YA1959, Rabbit)

HY-P82214
MedChem Express
TargetRPSA
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    67kDa Laminin Receptor Antibody (YA1959, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3921
  • Target name
    RPSA
  • Target description
    ribosomal protein SA
  • Target synonyms
    37LRP, 67LR, ICAS, LAMBR, LAMR1, LBP, LBP/p40, LRP, LRP/LR, NEM/1CHD4, SA, lamR, p40, uS2, small ribosomal subunit protein uS2, 37 kDa laminin receptor, 37/67 kDa laminin receptor, 40S ribosomal protein SA, 67 kDa laminin receptor, colon carcinoma laminin-binding protein, laminin receptor 1 (67kD, ribosomal protein SA), laminin-binding protein precursor p40, multidrug resistance-associated protein MGr1-Ag
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    67kDa Laminin Receptor Antibody (YA1959) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1959), targeting 67kDa Laminin Receptor, with a predicted molecular weight of 33 kDa (observed band size: 40 kDa). 67kDa Laminin Receptor Antibody (YA1959) can be used for WB, IP experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPSA using anti-RPSA antibody (PB10094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPSA antigen affinity purified polyclonal antibody (Catalog # PB10094) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPSA at approximately 40 kDa. The expected band size for RPSA is at 33 kDa.
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