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RPSA Polyclonal Antibody

CAC13914
Biomatik
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetRPSA
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Overview

  • Supplier
    Biomatik
  • Product Name
    RPSA Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3921
  • Target name
    RPSA
  • Target description
    ribosomal protein SA
  • Target synonyms
    37 kDa laminin receptor; 37/67 kDa laminin receptor; 37LRP; 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; colon carcinoma laminin-binding protein; ICAS; LAMBR; laminin receptor 1 (67kD, ribosomal protein SA); laminin-binding protein precursor p40; lamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; SA; small ribosomal subunit protein uS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08865
  • Protein Name
    40S ribosomal protein SA
  • Scientific Description
    The RPSA Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPSA using anti-RPSA antibody (PB10094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPSA antigen affinity purified polyclonal antibody (Catalog # PB10094) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPSA at approximately 40 kDa. The expected band size for RPSA is at 33 kDa.
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