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Western Blot analysis of Mouse heart tissue and 231 cell using AATF Polyclonal Antibody at dilution of 1:667
Western Blot analysis of Mouse heart tissue and 231 cell using AATF Polyclonal Antibody at dilution of 1:667
Western Blot analysis of Mouse heart tissue and 231 cell using AATF Polyclonal Antibody at dilution of 1:667

AATF Polyclonal Antibody

E-AB-16241
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    AATF Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Concentration
    0.9mg/ml
  • Scientific Description
    The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AATF using anti-AATF antibody (A03945-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody (Catalog # A03945-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AATF at approximately 95 kDa. The expected band size for AATF is at 63 kDa.
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