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Immunofluorescent analysis of A549 cells using CSB-PA001062LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of A549 cells using CSB-PA001062LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of A549 cells using CSB-PA001062LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ABCC3 Antibody

CSB-PA001062LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetABCC3
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Overview

  • Supplier
    Cusabio
  • Product Name
    ABCC3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8714
  • Target name
    ABCC3
  • Target description
    ATP binding cassette subfamily C member 3
  • Target synonyms
    ABC31; ATP-binding cassette sub-family C member 3; ATP-binding cassette, sub-family C (CFTR/MRP), member 3; canalicular multispecific organic anion transporter 2; canicular multispecific organic anion transporter; cMOAT2; EST90757; MLP2; MOAT-D; MRP3; multidrug resistance associated protein; multidrug resistance-associated protein 3; multi-specific organic anion transporter D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15438
  • Protein Name
    Canalicular multispecific organic anion transporter 2
  • Scientific Description
    May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP3/ABCC3 antigen affinity purified polyclonal antibody (Catalog # A02429-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP3/ABCC3 at approximately 200 kDa. The expected band size for MRP3/ABCC3 is at 169 kDa.
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