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ProductsBack/ATP Binding Cassette Transporter C3 (ABCC3) Polyclonal Antibody

ATP Binding Cassette Transporter C3 (ABCC3) Polyclonal Antibody

CAU23225
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetABCC3
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Overview

  • Supplier
    Biomatik
  • Product Name
    ATP Binding Cassette Transporter C3 (ABCC3) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB; ICC; IHC-P; IHC-F; ELISA.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8714
  • Target name
    ABCC3
  • Target description
    ATP binding cassette subfamily C member 3
  • Target synonyms
    ABC31, EST90757, MLP2, MOAT-D, MRP3, cMOAT2, ATP-binding cassette sub-family C member 3, ATP-binding cassette, sub-family C (CFTR/MRP), member 3, canalicular multispecific organic anion transporter 2, canicular multispecific organic anion transporter, multi-specific organic anion transporter D, multidrug resistance associated protein, multidrug resistance-associated protein 3
  • Host
    Rabbit
  • Protein IDO15438
  • Protein Name
    ATP-binding cassette sub-family C member 3
  • Scientific Description
    The ATP Binding Cassette Transporter C3 (ABCC3) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, ICC, IHC-P, IHC-F, ELISA.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP3/ABCC3 antigen affinity purified polyclonal antibody (Catalog # A02429-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP3/ABCC3 at approximately 200 kDa. The expected band size for MRP3/ABCC3 is at 169 kDa.
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