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ABCC3 / MRP3 Antibody

LS-C411370
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetABCC3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ABCC3 / MRP3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 55kDa/64kDa/137kDa/169kDa/174kDa, while the observed MW by Western blot was 169kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 55kDa/64kDa/137kDa/169kDa/174kDa, while the observed MW by Western blot was 169kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8714
  • Target name
    ABCC3
  • Target description
    ATP binding cassette subfamily C member 3
  • Target synonyms
    ABC31; ATP-binding cassette sub-family C member 3; ATP-binding cassette, sub-family C (CFTR/MRP), member 3; canalicular multispecific organic anion transporter 2; canicular multispecific organic anion transporter; cMOAT2; EST90757; MLP2; MOAT-D; MRP3; multidrug resistance associated protein; multidrug resistance-associated protein 3; multi-specific organic anion transporter D
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP3/ABCC3 antigen affinity purified polyclonal antibody (Catalog # A02429-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP3/ABCC3 at approximately 200 kDa. The expected band size for MRP3/ABCC3 is at 169 kDa.
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