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ABL1 Polyclonal Antibody

CAC14060
Biomatik
ApplicationsImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetABL1
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Overview

  • Supplier
    Biomatik
  • Product Name
    ABL1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25
  • Target name
    ABL1
  • Target description
    ABL proto-oncogene 1, non-receptor tyrosine kinase
  • Target synonyms
    ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl, tyrosine-protein kinase ABL1, ABL protooncogene 1 nonreceptor tyrosine kinase, Abelson tyrosine-protein kinase 1, BCR-ABL1 p190, BCR/ABL e8a2 fusion, BCR/ABL1 e1a2 fusion protein, BCR/ABL1 fusion, BCR/ABL1 fusion protein e3a1, BCR::ABL1 fusion protein, bcr/c-abl oncogene protein, c-abl oncogene 1, receptor tyrosine kinase, chimeric BCR::ABL1 protein, proto-oncogene c-Abl, proto-oncogene tyrosine-protein kinase ABL1, v-abl Abelson murine leukemia viral oncogene homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00519
  • Protein Name
    Tyrosine-protein kinase ABL1
  • Scientific Description
    The ABL1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABL1 using anti-ABL1 antibody (A00133-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABL1 antigen affinity purified polyclonal antibody (Catalog # A00133-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABL1 at approximately 143 kDa. The expected band size for ABL1 is at 133 kDa.
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