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ICC/IF analysis of methanol-fixed HeLa cells using GTX83254 ABL2 antibody [1H1B11]. Green : ABL2
ICC/IF analysis of methanol-fixed HeLa cells using GTX83254 ABL2 antibody [1H1B11]. Green : ABL2
ICC/IF analysis of methanol-fixed HeLa cells using GTX83254 ABL2 antibody [1H1B11]. Green : ABL2

ABL2 antibody [1H1B11]

GTX83254
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetABL2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ABL2 antibody [1H1B11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1H1B11
  • Conjugate
    Unconjugated
  • Gene ID27
  • Target name
    ABL2
  • Target description
    ABL proto-oncogene 2, non-receptor tyrosine kinase
  • Target synonyms
    Abelson tyrosine-protein kinase 2; abelson-related gene protein; ABLL; ARG; c-abl oncogene 2, non-receptor tyrosine kinase; tyrosine-protein kinase ABL2; tyrosine-protein kinase ARG; v-abl Abelson murine leukemia viral oncogene homolog 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP42684
  • Protein Name
    Tyrosine-protein kinase ABL2
  • Scientific Description
    This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABL2 using anti-ABL2 antibody (PB9913). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates, Lane 5: huamn K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABL2 antigen affinity purified polyclonal antibody (Catalog # PB9913) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABL2 at approximately 140 kDa. The expected band size for ABL2 is at 128 kDa.
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