Ac-ANW-AMC is a fluorogenic peptide substrate for measuring chymotrypsin-like activity of the immunoproteasome. Hydrolysis of this substrate by the beta5i-subunit of the immunoproteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the immunoproteasome and is not hydrolyzed efficiently by the constitutive proteasome. - Chemical. CAS: n/a. Formula: C30H32N6O7. MW: 588.6. Ac-ANW-AMC is a fluorogenic peptide substrate for measuring chymotrypsin-like activity of the immunoproteasome. Hydrolysis of this substrate by the beta5i-subunit of the immunoproteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the immunoproteasome and is not hydrolyzed efficiently by the constitutive proteasome.