Sample (30 μg of whole cell lysate) A: whole zebrafish 10% SDS PAGE GTX102637 diluted at 1:1000
![ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102637/GTX102637_39764_IFA_w_23060119_560.webp "ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Blue: Hoechst 33342 staining.")
![ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102637/GTX102637_39764_20150410_IFA_w_23060119_279.webp "ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.")
![ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis. Sample: Paraffin-embedded human normal kidney. ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102637/GTX102637_39764_20141128_IHC_w_23060119_573.webp "ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis. Sample: Paraffin-embedded human normal kidney. ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
Sample (30 μg of whole cell lysate) A: whole zebrafish 10% SDS PAGE GTX102637 diluted at 1:1000
ACAT1 antibody [N1N3]
GTX102637
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Zebra Fish
TargetACAT1
Overview
- SupplierGeneTex
- Product NameACAT1 antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID38
- Target nameACAT1
- Target descriptionacetyl-CoA acetyltransferase 1
- Target synonymsACAT, MAT, T2, THIL, acetyl-CoA acetyltransferase, mitochondrial, acetoacetyl Coenzyme A thiolase, acetoacetyl-CoA thiolase, acetyl-Coenzyme A acetyltransferase 1, mitochondrial acetoacetyl-CoA thiolase, testicular tissue protein Li 198
- HostRabbit
- IsotypeIgG
- Protein IDP24752
- Protein NameAcetyl-CoA acetyltransferase, mitochondrial
- Scientific DescriptionThis gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq]
- ReactivityHuman, Mouse, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Yano H, Fujiwara Y, Horlad H, et al. Blocking cholesterol efflux mechanism is a potential target for antilymphoma therapy. Cancer Sci. 2022,113(6):2129-2143. doi: 10.1111/cas.15349Read this paper
- Chimalapati S, de Souza Santos M, Lafrance AE, et al. Vibrio deploys type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate cell egress. Elife. 2020,9. doi: 10.7554/eLife.58057Read this paper
- Chen JF, Tsai YT, Lai YH, et al. Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells. J Pharm Biomed Anal. 2020,187:113142. doi: 10.1016/j.jpba.2020.113142Read this paper
- Lo YW, Lin ST, Chang SJ, et al. Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer. J Cell Mol Med. 2015,19(4):744-59. doi: 10.1111/jcmm.12388Read this paper
- Kato K, Yazawa T, Taki K, et al. The inositol 5-phosphatase SHIP2 is an effector of RhoA and is involved in cell polarity and migration. Mol Biol Cell. 2012,23(13):2593-604. doi: 10.1091/mbc.E11-11-0958Read this paper
Datasheet
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