
Sample (30 μg of whole cell lysate) A: whole zebrafish 10% SDS PAGE GTX102637 diluted at 1:1000
ACAT1 antibody [N1N3]
GTX102637
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Zebra Fish
TargetACAT1
Overview
- SupplierGeneTex
- Product NameACAT1 antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID38
- Target nameACAT1
- Target descriptionacetyl-CoA acetyltransferase 1
- Target synonymsACAT, MAT, T2, THIL, acetyl-CoA acetyltransferase, mitochondrial, acetoacetyl Coenzyme A thiolase, acetoacetyl-CoA thiolase, acetyl-Coenzyme A acetyltransferase 1, mitochondrial acetoacetyl-CoA thiolase, testicular tissue protein Li 198
- HostRabbit
- IsotypeIgG
- Protein IDP24752
- Protein NameAcetyl-CoA acetyltransferase, mitochondrial
- Scientific DescriptionThis gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq]
- ReactivityHuman, Mouse, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Blue: Hoechst 33342 staining. ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102637/GTX102637_39764_IFA_w_23060119_560.webp)
![ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining. ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102637/GTX102637_39764_20150410_IFA_w_23060119_279.webp)


![ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis. Sample: Paraffin-embedded human normal kidney. ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis. Sample: Paraffin-embedded human normal kidney. ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102637/GTX102637_39764_20141128_IHC_w_23060119_573.webp)





