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Western blot All lanes: ACAT1 antibody at 6microg/ml Lane 1: Mouse skeletal musce tissue Lane 2: Rat heart tissue Lane 3: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46, 18 kDa Observed band size: 46 kDa
Western blot All lanes: ACAT1 antibody at 6microg/ml Lane 1: Mouse skeletal musce tissue Lane 2: Rat heart tissue Lane 3: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46, 18 kDa Observed band size: 46 kDa
Western blot All lanes: ACAT1 antibody at 6microg/ml Lane 1: Mouse skeletal musce tissue Lane 2: Rat heart tissue Lane 3: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46, 18 kDa Observed band size: 46 kDa

ACAT1 Antibody

CSB-PA001134ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetACAT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACAT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID38
  • Target name
    ACAT1
  • Target description
    acetyl-CoA acetyltransferase 1
  • Target synonyms
    ACAT; acetoacetyl Coenzyme A thiolase; acetoacetyl-CoA thiolase; acetyl-CoA acetyltransferase, mitochondrial; acetyl-Coenzyme A acetyltransferase 1; MAT; mitochondrial acetoacetyl-CoA thiolase; T2; testicular tissue protein Li 198; THIL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24752
  • Protein Name
    Acetyl-CoA acetyltransferase, mitochondrial
  • Scientific Description
    Plays a major role in ketone body metabolism.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody HPA004428 (A) shows similar protein distribution across tissues to independent antibody HPA007569 (B).
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Figure 1. Western blot analysis of ACAT1 using anti-ACAT1 antibody (A02008-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAT1 antigen affinity purified polyclonal antibody (Catalog # A02008-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAT1 at approximately 40 kDa. The expected band size for ACAT1 is at 40 kDa.
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