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ProductsBack/ACE2 Antibody - middle region (ARP53751_P050)

ACE2 Antibody - middle region (ARP53751_P050)

ORB582208
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
TargetACE2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    ACE2 Antibody - middle region (ARP53751_P050)
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID59272
  • Target name
    ACE2
  • Target description
    angiotensin converting enzyme 2
  • Target synonyms
    ACEH, angiotensin-converting enzyme 2, ACE-related carboxypeptidase, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin I converting enzyme 2, angiotensin-converting enzyme homolog, angiotensin-converting enzyme-related carboxypeptidase, metalloprotease MPROT15, peptidyl-dipeptidase A
  • Host
    Rabbit
  • Protein IDQ9BYF1
  • Protein Name
    Angiotensin-converting enzyme 2
  • Scientific Description
    Rabbit polyclonal antibody to ACE2
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Gultom M, Licheri M, Laloli L, et al. Susceptibility of Well-Differentiated Airway Epithelial Cell Cultures from Domestic and Wild Animals to Severe Acute Respiratory Syndrome Coronavirus 2. Emerg Infect Dis. 2021,27(7):1811-1820. doi: 10.3201/eid2707.204660
    Read this paper

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Figure 1. Western blot analysis of ACE2 using anti-ACE2 antibody (A00756-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE2 antigen affinity purified polyclonal antibody (Catalog # A00756-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE2 at approximately 120 kDa. The expected band size for ACE2 is at 93 kDa.
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