IHC-P analysis of human kidney tissue section using GTX01160 ACE2 antibody [SN0754].
![Human tissue extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01160/GTX01160_HK0921_20200228_WB__kidney_w_23053121_752.webp "Human tissue extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX01160/GTX01160_HK0921_20200313_IHC-P_1_w_23053121_372.webp "ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX01160/GTX01160_HK0921_20200313_IHC-P_2_w_23053121_729.webp "ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human intestine. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX01160/GTX01160_HK0921_20200313_IHC-P_3_w_23053121_699.webp "ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human intestine. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX01160/GTX01160_HK0921_20200313_IHC-P_M_w_23053121_166.webp "ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX01160/GTX01160_HK0921_20200313_IHC-P_R_w_23053121_553.webp "ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Mouse tissue extract (50 microg) was separated by 10% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01160/GTX01160_HK0921_20200313_WB_M_kidney_w_23053121_195.webp "Mouse tissue extract (50 microg) was separated by 10% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunofluorescent analysis. Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min. Green: ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 microm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01160/GTX01160_HK0921_20200610_ICC_IF_Africangreenmonkey_w_23053121_654.webp "ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunofluorescent analysis. Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min. Green: ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 microm.")
![Untreated (-) and treated (+) Vero E6 whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01160/GTX01160_HK0921_20200814_WB_treatment_Tunicamycin_w_23053121_329.webp "Untreated (-) and treated (+) Vero E6 whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
IHC-P analysis of human kidney tissue section using GTX01160 ACE2 antibody [SN0754].
ACE2 antibody [SN0754]
GTX01160
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetACE2
Overview
- SupplierGeneTex
- Product NameACE2 antibody [SN0754]
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSN0754
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID59272
- Target nameACE2
- Target descriptionangiotensin converting enzyme 2
- Target synonymsACEH; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; angiotensin-converting enzyme 2; angiotensin-converting enzyme homolog; angiotensin-converting enzyme-related carboxypeptidase; metalloprotease MPROT15; peptidyl-dipeptidase A; truncated angiotensin converting enzyme 2
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThe protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Monkey, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- SARS-CoV-2 down-regulates ACE2 through lysosomal degradation. Lu Y et al., 2022 Dec 1, Mol Biol CellRead more
- Identification of cell type specific ACE2 modifiers by CRISPR screening. Sherman EJ et al., 2022 Mar, PLoS PathogRead more
- Heterogeneous expression of ACE2 and TMPRRS2 in mesenchymal stromal cells. Generali M et al., 2022 Jan, J Cell Mol MedRead more
- Tumor Necrosis Factor-Alpha Exacerbates Viral Entry in SARS-CoV2-Infected iPSC-Derived Cardiomyocytes. Lee CY et al., 2021 Sep 13, Int J Mol SciRead more
- Small Structural Proteins E and M Render the SARS-CoV-2 Pseudovirus More Infectious and Reveal the Phenotype of Natural Viral Variants. Wang HI et al., 2021 Aug 23, Int J Mol SciRead more
- ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomes. Sherman EJ et al., 2021 Aug 5, Sci RepRead more
- Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles. Hsieh MH et al., 2021, Front ImmunolRead more
- Expression of Endogenous Angiotensin-Converting Enzyme 2 in Human Induced Pluripotent Stem Cell-Derived Retinal Organoids. Ahmad Mulyadi Lai HI et al., 2021 Jan 28, Int J Mol SciRead more
- SARS-CoV-2 infects cells after viral entry via clathrin-mediated endocytosis. Bayati A et al., 2021 Jan-Jun, J Biol ChemRead more
- Targeting transcriptional regulation of SARS-CoV-2 entry factors ACE2 and TMPRSS2. Qiao Y et al., 2020 Dec 11, Proc Natl Acad Sci U S ARead more