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ACHE Antibody (YA3883, Mouse)

HY-P84186
MedChem Express
TargetACHE
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ACHE Antibody (YA3883, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID43
  • Target name
    ACHE
  • Target description
    acetylcholinesterase (Yt blood group)
  • Target synonyms
    ACEE, ARACHE, N-ACHE, YT, acetylcholinesterase, Yt blood group, acetylcholinesterase (Cartwright blood group), apoptosis-related acetylcholinesterase
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    ACHE Antibody (YA3883) is a mouse-derived and non-conjugated IgG1 antibody, targeting to ACHE. It can be applicated for WB, IHC-P, FC assays, in the background of human, mouse, monkey, rat.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACHE using anti-ACHE antibody (PB9417). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: PANC Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACHE antigen affinity purified polyclonal antibody (Catalog # PB9417) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACHE at approximately 68 kDa. The expected band size for ACHE is at 68 kDa.
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