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WB analysis of various samples using GTX66702 AChR alpha 3 antibody. Lane1 : The Brain tissue lysate of Mouse(40ug) Lane2 : C6 whole cell lysate(40ug) Lane3 : A549 whole cell lysate(40ug) Lane4 : Hela whole cell lysate(40ug) Dilution : 1:500
WB analysis of various samples using GTX66702 AChR alpha 3 antibody. Lane1 : The Brain tissue lysate of Mouse(40ug) Lane2 : C6 whole cell lysate(40ug) Lane3 : A549 whole cell lysate(40ug) Lane4 : Hela whole cell lysate(40ug) Dilution : 1:500
WB analysis of various samples using GTX66702 AChR alpha 3 antibody. Lane1 : The Brain tissue lysate of Mouse(40ug) Lane2 : C6 whole cell lysate(40ug) Lane3 : A549 whole cell lysate(40ug) Lane4 : Hela whole cell lysate(40ug) Dilution : 1:500

AChR alpha 3 antibody

GTX66702
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCHRNA3
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Overview

  • Supplier
    GeneTex
  • Product Name
    AChR alpha 3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1136
  • Target name
    CHRNA3
  • Target description
    cholinergic receptor nicotinic alpha 3 subunit
  • Target synonyms
    BAIPRCK; cholinergic receptor, nicotinic alpha 3; cholinergic receptor, nicotinic, alpha 3 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 3; LNCR2; NACHRA3; neuronal acetylcholine receptor subunit alpha-3; neuronal nicotinic acetylcholine receptor, alpha3 subunit; PAOD2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32297
  • Protein Name
    Neuronal acetylcholine receptor subunit alpha-3
  • Scientific Description
    This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHRNA3 using anti-CHRNA3 antibody (A01981-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: human PANC-1 whole cell lysates, Lane 7: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRNA3 antigen affinity purified polyclonal antibody (Catalog # A01981-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRNA3 at approximately 60KD. The expected band size for CHRNA3 is at 57KD.
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