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Acidic Cytokeratin antibody [AE1]

GTX80320
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKRT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Acidic Cytokeratin antibody [AE1]
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AE1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3848
  • Target name
    KRT1
  • Target description
    keratin 1
  • Target synonyms
    67 kDa cytokeratin; CK1; CK-1; cytokeratin 1; EHK; EHK1; epidermolytic hyperkeratosis 1; EPPK; hair alpha protein; K1; keratin 1, type II; keratin, type II cytoskeletal 1; KRT1A; NEPPK; type-II keratin Kb1
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Characterization of immortalized human epidermolysis bullosa simplex (KRT5) cell lines: trimethylamine N-oxide protects the keratin cytoskeleton against disruptive stress condition. Chamcheu JC et al., 2009 Mar, J Dermatol Sci
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Figure 1. Western blot analysis of Cytokeratin 1/KRT1 using anti-Cytokeratin 1/KRT1 antibody (A01639). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 1/KRT1 antigen affinity purified polyclonal antibody (Catalog # A01639) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 1/KRT1 at approximately 66 kDa. The expected band size for Cytokeratin 1/KRT1 is at 66 kDa.
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