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Anti-HMW Cytokeratin Antibody

188-10212
RayBiotech
TargetKRT1
Product group Antibodies
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-HMW Cytokeratin Antibody
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Concentration
    0.2 mg/ml
  • Gene ID3848
  • Target name
    KRT1
  • Target description
    keratin 1
  • Target synonyms
    67 kDa cytokeratin; CK1; CK-1; cytokeratin 1; EHK; EHK1; epidermolytic hyperkeratosis 1; EPPK; hair alpha protein; K1; keratin 1, type II; keratin, type II cytoskeletal 1; KRT1A; NEPPK; type-II keratin Kb1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04264
  • Protein Name
    Keratin, type II cytoskeletal 1
  • Scientific Description
    Mouse anti-Human/Mouse/Rat HMW Cytokeratin Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytokeratin 1/KRT1 using anti-Cytokeratin 1/KRT1 antibody (A01639). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 1/KRT1 antigen affinity purified polyclonal antibody (Catalog # A01639) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 1/KRT1 at approximately 66 kDa. The expected band size for Cytokeratin 1/KRT1 is at 66 kDa.
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