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ACKR3 / CXCR7 Antibody

LS-C747794
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACKR3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACKR3 / CXCR7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 41kDa, while the observed MW by Western blot was 55kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 41kDa, while the observed MW by Western blot was 55kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57007
  • Target name
    ACKR3
  • Target description
    atypical chemokine receptor 3
  • Target synonyms
    CMKOR1, CXC-R7, CXCR-7, CXCR7, GPR159, RDC-1, RDC1, atypical chemokine receptor 3, C-X-C chemokine receptor type 7, G protein-coupled receptor, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog, chemokine (C-X-C motif) receptor 7, chemokine orphan receptor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GPCR RDC1/CXCR-7/ACKR3 using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR RDC1/CXCR-7/ACKR3 antigen affinity purified polyclonal antibody (Catalog # A02656-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR RDC1/CXCR-7/ACKR3 at approximately 41 kDa. The expected band size for GPCR RDC1/CXCR-7/ACKR3 is at 41 kDa.
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